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Brand Name : NANBEI
Certification : ISO,SGS,CE
Place of Origin : China
MOQ : 50/set
Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram
Delivery Time : 5-7days
Model Number : Cell Insert Multiple Well Plate
Supply Ability : 500 set/per day
Packaging Details : Wooden cases and cartons
packing specification : 6
Inserts/plate : 6 inserts/6well
Insert Diameter(mm) : 24
Well Volume(ml) : 2.6
Insert Volume(ml) : 1.5
Name : Cell Insert Multiple Well Plate
Multiple well plate or dish is made from PS
 
 Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.
 This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
 Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.
 
 ·Innovative hanging design facilitates pipetting.
 ·Supplied in multiple well plates: 6-well, 12-well, 24well.
 ·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
 ·Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
 · Transparent PET membrane is easy to observe.
 ·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
 ·Compatible with most solvents used for fixing and dyeing.
 
 Attention:
 • Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
 •During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
 •Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.
 
 Guidelines for us:
 • Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
 • Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
 • Check the volume of the culture medium regularly and add fresh medium as needed.
 • The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
 • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
 Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
| packing specification | Inserts/plate | Insert Diameter(mm) | Well Volume(ml) | Insert Volume(ml) | Insert Membrane Growth Area(cm²) | 
| 6 | 6 inserts/6well | 24 | 2.6 | 1.5 | 4.67 | 
| 12 | 12 inserts/6well | 12 | 1.5 | 0.5 | 1.12 | 
| 24 | 12 inserts/6well | 6.5 | 0.6 | 0.1 | 0.33 | 
| 100mm dish | 1 inserts/6well | 75 | 13 | 9 | 44 | 

| Pore Size(um) | Membrane Density(well/cm²) | 
| 0.4 | 1x108 | 
| 3.0 | 2x106 | 
| 5.0 | 4x105 | 
| 8.0 | 1x105 | 
 50% Transparent PC Membrane Cell Insert, Tissue Culture Treated(Individually packaged in peelable Tyvek film and Blister,10/case)
| Cat.No. | Type(Well) | Pore Size(um) | 
| 723001 | 6 | 3.0 | 
| 724001 | 12 | 3.0 | 
| 725001 | 24 | 3.0 | 
| 723101 | 6 | 0.4 | 
| 724101 | 12 | 0.4 | 
| 725101 | 24 | 0.4 | 
| 724201 | 12 | 5.0 | 
| 725201 | 24 | 5.0 | 
| 723301 | 6 | 8.0 | 
| 724301 | 12 | 8.0 | 
| 725301 | 24 | 8.0 | 
| 726001 | 100mm dish | 3.0 | 

 50% Transparent PC Membrane Cell Insert, Non-treated(Individually packaged in peelable Tyvek film and Blister,10/case)
| Cat.No. | Type(Well) | Pore Size(um) | 
| 723011 | 6 | 3.0 | 
| 724011 | 12 | 3.0 | 
| 725011 | 24 | 3.0 | 
| 723111 | 6 | 0.4 | 
| 724111 | 12 | 0.4 | 
| 725111 | 24 | 0.4 | 
| 724211 | 12 | 5.0 | 
| 725211 | 24 | 5.0 | 
| 723311 | 6 | 8.0 | 
| 724311 | 12 | 8.0 | 
| 725311 | 24 | 8.0 | 
 Transparent PET Membrane Cell Insert, Tissue Culture Treated(Individually packaged in peelable Tyvek film and Blister,10/case)
| Cat.No. | Type(Well) | Pore Size(um) | 
| 723121 | 6 | 0.4 | 
| 724121 | 12 | 0.4 | 
| 725121 | 24 | 0.4 | 
| 723021 | 6 | 3.0 | 
| 724021 | 12 | 3.0 | 
| 725021 | 24 | 3.0 | 

 Transparent PET Membrane Cell Insert, Non-treated(Individually packaged in peelable Tyvek film and Blister,10/case)
| Cat.No. | Type(Well) | Pore Size(um) | 
| 723131 | 6 | 0.4 | 
| 724131 | 12 | 0.4 | 
| 725131 | 24 | 0.4 | 
| 723031 | 6 | 3.0 | 
| 724031 | 12 | 3.0 | 
| 725031 | 24 | 3.0 | 
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